Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: IGF-PLGA microspheres promote angiogenesis and accelerate skin flap repair and healing by inhibiting oxidative stress and regulating the Ang 1/Tie 2 signaling pathway.

doi: 10.1016/j.ejps.2023.106687

Figure Lengend Snippet: Fig. 7. IGF-PLGA microspheres inhibit T-BHP induced oxidative stress in 3T3 cells.(A)Western blot of HO-1,SOD1 and SOD2 in different groups.(B,C,D)The statistical chart shows the protein expression levels of HO-1,SOD1 and SOD2. Our experimental data is reported as mean ± SD. * p<0.05, * * p<0.01 vs T-BHP group.(E)HO-1 immunofluorescence in different groups, scale bar=20 μM.(F) The statistical chart shows the average fluorescence intensity of HO-1. Our experimental data is re ported as mean ± SD. * p<0.05, * * p<0.01 vs T-BHP group.

Article Snippet: Primary antibodies to superoxide dismutase 1 (SOD1, Cat No.10269-1-AP), SOD2 (Cat No. 24,127–1-AP), HO-1 (Cat No. 66743–1-Ig), proliferating cell nuclear antigen (PCNA, Cat No. 60097–1-Ig), Bcl-2-associated x (Bax, Cat No. 50599–2-Ig), B-cell lymphoma-2 (Bcl-2, Cat No. 68103–1-Ig), Caspase 3 (Cat No. 66470–2- Ig) were purchased from Proteintech group.

Techniques: Western Blot, Expressing, Immunofluorescence, Fluorescence